Figure 3
Effect of an inhibitor specific to 5-HT7 receptor (‘SB-269970’) on hemocyte behaviors of S. exigua. (a) Total hemocyte count (THC) analysis in L5 larvae. For the assay, 2 µg of 5-HT or 2 µg of SB-269970 was injected. THC was assessed with or without bacterial co-injection (1.8 × 105 cells per larva). (b) Hemocyte-spreading behavior analysis. Hemocyte-spreading was assessed with a 20 µL reaction mixture containing 1 or 2 µL test chemical with 18 or 19 µL hemocytes. 5-HT was co-applied with SB-269970 at ratios of 1:10 (2 µg 5-HT: 20 µg SB-269970) and 10:1 (20 µg 5-HT: 2 µg SB-269970). Spread cells were stained with F-actin and FITC-labeled phalloidin. Nuclei were stained with DAPI. Each treatment was independently replicated three times. Histogram bars indicate percentages of spread hemocytes and error bars indicate standard deviation. Histogram bars annotated with the same letter are not significantly different at Type I error = 0.05 (LSD test).
