Figure 9 | Scientific Reports

Figure 9

From: Phenylethylamides derived from bacterial secondary metabolites specifically inhibit an insect serotonin receptor

Figure 9The alternative text for this image may have been generated using AI.

Competitive inhibition of 10 selected bacterial metabolites derived from X. nematophila and P. temperata temperata with 5-HT against Se-5HTR mediating phagocytosis. FITC-tagged E. coli cells were injected to L5 larvae along with test chemicals. After 15 min, phagocytic cells were observed under a fluorescent microscope at 400 × magnification and percentage of phagocytotic hemocytes was calculated from randomly chosen 100 cells. Each treatment was independently replicated three times. (a) Dose-response of inhibitory compound (0, 0.01, 0.1, 1, and 10 µg/larva) with a fixed 5-HT concentration (1 µg/larva). (b) Dose-response of 5-HT (0, 0.02, 0.2, 2 and 20 µg/larva) with a fixed HB compound concentration (1 µg/larva). ‘CON’ represents a positive control without any inhibitor.

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