Figure 4
Disruption of CXCR6 inhibits fibronectin and collagen I expression. (A) The mRNA levels of fibronectin (FN) in the kidney of WT and CXCR6 KO mice in response to DOCA-salt. **P < 0.01 vs WT-CON, +P < 0.05 vs KO-DOCA, and #P < 0.05 vs WT-DOCA. n = 3–4 per group. (B) The mRNA levels of collagen I in the kidney of WT and CXCR6 KO mice in response to DOCA-salt. **P < 0.01 vs WT-CON, ++P < 0.01 vs KO-DOCA, and #P < 0.05 vs WT-DOCA. n = 4 per group. (C) Representative photomicrographs of kidney sections stained for fibronectin (green) and counterstained with DAPI (blue). Scale bar, 50 μm. (D) Quantitative analysis of fibronectin-positive area in the kidneys. **P < 0.01 vs WT-CON, ++P < 0.01 vs KO-DOCA, #P < 0.05 vs WT-DOCA. n = 6 in each group. (E) Representative photomicrographs of kidney sections stained for collagen I (green) and counterstained with DAPI (blue). Scale bar, 50 μm. (F) Quantitative analysis of collagen I-positive area in the kidneys. **P < 0.01 vs WT-CON, ++P < 0.01 vs KO-DOCA, #P < 0.05 vs WT-DOCA. n = 6 in each group.
