Figure 8

Expression of CCL19 and CXCL13 proteins by 3D-cultured spleen cells upon LTβR stimulation. (a) Representative images of Venus and CCL19 expression by cells from the spleen of Tlx1CreER-Venus mice (P7) under the unstimulated condition. Scale bars indicate 30 μm. (b) Representative images of Venus and CCL19 expression by cells after LTβR stimulation for 48 hours. (c) Representative images of Venus and CXCL13 expression by cells after LTβR stimulation. (d) Frequencies of CCL19+ cells in Venus+ and Venus− cell populations under unstimulated (650 cells from 5 independent experiments) and stimulated (366 cells from 4 independent experiments) conditions. (mean ± SD). (e) Frequencies of CXCL13+ cells in Venus+ and Venus− cell populations under unstimulated (722 cells from 5 independent experiments) and stimulated (514 cells from 4 independent experiments) conditions. (mean ± SD).