Figure 5
Exo-WT and Exo-C increase and Exo-1537S decrease tumorigenic capacity in an in vivo breast cancer model of peritoneal carcinomatosis. (A) BalbC NOD/SCID mice were injected ip with 2,5 × 106 MDA-MB-231 cells in saline alone (250 µl) or containing 10 µg Exo-WT, Exo-C or Exo-1537S (7 mice per group). A separate control group of 6 mice was left uninoculated. Every three days, the animals were injected ip with 10 µg of the corresponding exosome preparation, or saline and mice were euthanized on day 21 post-inoculation. The rating of metastasis was evaluated in tumor-mesentery, spleen and by formation of malignant ascites. (B) Total weight of tumor-mesenteric tissue. (C) Total weight of spleen/pancreas. Statistically significant differences are indicated *p < 0.05, **p < 0.01, ***p < 0.001. Photographs of representative experiments showing tumor growth in these tissues are displayed in the lower part of each graph. (D) Photographs of representative mice from each group showing comparison in abdominal width, total mouse weight and ascites volume. (E) Quantification of cells/ml in ascitic fluid. Statistically significant differences are indicated *p < 0.05, **p < 0.01, ***p < 0.001, Tukey Multicomparison assays.
