Figure 3 | Scientific Reports

Figure 3

From: The N-terminal Acetylation of α-Synuclein Changes the Affinity for Lipid Membranes but not the Structural Properties of the Bound State

Figure 3

INEPT-based identification of dynamical regions of NTAc-αS bound to DOPE:DOPS:DOPC SUVs. 13C-detected 1H-13C correlation via INEPT transfer (a) and 1H-detected 1H-15N-HSQC (b) of NTAc-αS bound to DOPE:DOPS:DOPC SUVs recorded at 4 °C using MAS ssNMR with spinning rate of 10 kHz. The experiments were performed at a 1H Larmor frequency of 700 MHz using a 3.2 mm EFree probe. Atom names ca, cb, cg, cd are used for Cα, Cβ, Cγ and Cδ atoms, respectively. Assignment of the spectra were derived from previous studies of vesicle bound αS44.

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