Figure 1
From: Establishment of sperm cryopreservation and in vitro fertilisation protocols for rats
Motile parameters of fresh and cryopreserved rat sperm Parameters of sperm motility were analysed by a computer-assisted sperm analyser. (A) Motility is the ratio of sperm moving 5 μm/s to total sperm. (B) Progressive motility is the ratio of sperm moving at 50 μm/s and with straightness greater than 50% to total sperm. (C) Path velocity (VAP) is the average velocity of the smoothed sperm path. (D) Progressive velocity (VSL) is the average velocity measured in a straight line from the beginning to the end of the sperm track. (E) Lateral amplitude (ALH) is the mean width of the head oscillation as the sperm swims. (F) Beat frequency (BCF) is the frequency of sperm heads crossing the average sperm path in either direction. (G) Straightness (STR) is a measure of the departure of the average sperm path from a straight line (ratio of VSL/VAP). Values are given as the mean ± SD (n = 4). *p < 0.05 compared with fresh sperm.
