Table 1 Fertilisation rate of oocytes inseminated using fresh or frozen rat sperm.

From: Establishment of sperm cryopreservation and in vitro fertilisation protocols for rats

Sperm

No.

No. ofinseminated oocytes

No. offertilised oocytes

Fertilisation rate (%)

Fresh

1

198

185

93.4

2

183

170

92.9

3

195

181

92.8

4

182

174

95.6

Total

758

710

93.7 ± 1.3

Frozen

1

161

135

83.9

2

191

160

83.8

3

201

161

80.1

4

196

159

81.1

Total

749

615

82.1 ± 1.9*

  1. *P < 0.05 compared with fresh sperm.