Table 3 In vivo developmental ability of fertilised oocytes derived from fresh or frozen rat sperm.
From: Establishment of sperm cryopreservation and in vitro fertilisation protocols for rats
Sperm | No. | fertilised oocytes transferred | No. of pups | Birth rate (%) | No. of pups with GFP | Success rate (%) |
|---|---|---|---|---|---|---|
Fresh | 1 | 20 | 13 | 65.0 | 6 | 46.2 |
2 | 20 | 12 | 60.0 | 5 | 41.7 | |
3 | 20 | 13 | 65.0 | 6 | 46.2 | |
4 | 20 | 10 | 50.0 | 7 | 70.0 | |
Total | 80 | 48 | 60 ± 7.1 | 24 | 50.0 ± 12.8 | |
Frozen | 1 | 20 | 12 | 60.0 | 8 | 66.7 |
2 | 20 | 12 | 60.0 | 7 | 58.3 | |
3 | 20 | 10 | 50.0 | 2 | 20.0 | |
4 | 20 | 12 | 60.0 | 9 | 75.0 | |
Total | 80 | 46 | 57.5 ± 5.0 | 26 | 56.5 ± 24.3 |
