Figure 4

Optimal number of reference genes according to GenEx analyses. Accumulated standard deviation (Acc.SD) for the five candidate housekeeping genes in all groups of samples allows to estimate the ideal number of genes for normalization.Acc.SD was calculated with the GenEx software package. Lower values of Acc.SD indicate the optimal number of reference genes. CY, cystic; NC, non-cystic; HT, Pkd1-haploinsufficient; WT, wild-type; SC, severely cystic phenotype; CO, severely cystic phenotype controls.