Figure 6
The ΔCt values of miR-21 (A–C), let-7a (D–F) and miR-17 (G–I) target genes normalized by different combinations of the three best candidate housekeeping genes (miR-20a, miR-25 and miR-26a). A lower threshold value (Ct) indicates a higher gene expression. The median values are represented as horizontal lines, and the error bars represent interquartile range. CY, cystic; NC, non-cystic; HT, haploinsufficient; WT, wild-type; SC, severely cystic phenotype; CO, severely cystic phenotype controls. 20a, target expression normalized by miR-20a; 25, target expression normalized by miR-25; 26a, target expression normalized by miR-26a; 25 + 26a, target expression normalized by miR-25 + miR-26a; 20a + 25, target expression normalized by miR-20a + miR-25; 20a + 26a, target expression normalized by miR-20a + miR-26a, target expression normalized by miR-20a + miR-25 + miR-26a. *p < 0.008 by Mann-Whitney test, followed by Bonferroni correction.
