Table 2 Best combination of housekeeping miRNAs for each group of samples yielded by software analyses.
Groups | Best pair of housekeeping genes by software | Best pair of housekeeping gene by visual inspection | Best trio of housekeeping genes by visual inspection | |||||
|---|---|---|---|---|---|---|---|---|
NormFinder | GeNorm | RefFinder | ΔCt method | Bestkeeper | DataAssist | |||
All | miR-25 + miR-26a | miR-20a + miR-26a | miR-20a + miR-26a | miR-20a + miR-26a | miR-26a + miR-191 | miR-20a + miR-26a | miR-20a + miR-26a | miR-20a + miR-25 + miR-26a or miR-20a + miR-26a + miR-191 |
CY | miR-20a + miR-26a | miR-20a + miR-25 | miR-20a + miR-25 | miR-20a + miR-25 | miR-25 + miR-191 | miR-20a + miR-25 | miR-20a + miR-25 | miR-20a + miR-25 + miR-26a or miR-20a + miR-26a + miR-191 |
NC | miR-20a + miR-26a | miR-20a + miR-26a | miR-20a + miR-26a | miR-20a + miR-26a | miR-20a + miR-191 | miR-20a + miR-26a | miR-20a + miR-26a | miR-20a + miR-26a + miR-191 |
HT | miR-20a + miR-26a | miR-20a + miR-26a | miR-20a + miR-26a | miR-20a + miR-26a | miR-20a + miR-191 | miR-20a + miR-26a | miR-20a + miR-26a | miR-20a + miR-26a + miR-191 |
WT | miR-20a + miR-26a | miR-20a + miR-25 | miR-25 + miR-26a | miR-25 + miR-26a | miR-20a + miR-25 | miR-20a + miR-25 | miR-20a + miR-25 | miR-20a + miR-25 + miR-26a |
SC | miR-25 + miR-191 | miR-25 + miR-191 | miR-25 + miR-191 | miR-25 + miR-191 | miR-25 + miR-191 | miR-25 + miR-191 | miR-25 + miR-191 | — |
CO | miR-20a + U6 | miR-26a + miR-191 | miR-20a + miR-26a | miR-20a + miR-26a | U6 + miR-25 | miR-20a + miR-26a | miR-20a + miR-26a | miR-20a + miR-25 + miR-26a or miR-20a + miR-26a + miR-191 or miR-20a + miR-26a + U6 |
