Figure 2
From: Investigation of The Cellular Response to Bone Fractures: Evidence for Flexoelectricity
Osteocytes’ reaction to mechanical stimulation of already existing cracks. (A) Brightfield optical micrograph of the crack prior to culture. (B) Live/Dead staining image of the culture on the crack before bending the substrate. (C) Live/Dead staining image after bending the substrate. (D) Cell viability quantification after osteocytes stimulation by MTT assay versus not stimulated samples. Mechanical stimulation barely affects cell viability when there is no crack (‘stimulated - crack’) whereas it has a strong effect when there is a crack (‘stimulated + crack’). (N = 6). *P < 0.05.
