Figure 2
Photocycle of wild-type RxR. (A) Time-resolved difference absorption spectra in NaCl solution at 25 °C. (B) Time-resolved absorption changes in NaCl solution at 25 °C; the fitting curves are shown as gray. (Inset) Absorption changes of pyranine monitored at 450 nm; the fitting curve is shown as gray. (C) Photocycle model with the timing of uptake and release of the proton. (D) Arrhenius plots of the rate constants for the transition processes from the L- to M-intermediate (red circles), from the M- to O-intermediate (blue circles), and from the O-intermediate to original state (green circles). The data were analyzed by the Eyring equation to calculate the activation energies (Ea) listed in Table 1. The fitting curves are shown as black lines.
