Figure 5 | Scientific Reports

Figure 5

From: Vectorial Proton Transport Mechanism of RxR, a Phylogenetically Distinct and Thermally Stable Microbial Rhodopsin

Figure 5

Mutational effects of Asp85 and Glu187/Glu197 located in the intracellular and extracellular sides of RxR, respectively. (A) Time-resolved absorption changes of D85N in NaCl solution at 25 °C; the fitting curves are shown as gray. (B–D) Time-resolved absorption changes of E187Q (B), E197Q (C) and E187Q/E197Q (D) in NaCl solution at 25 °C. (E) Absorption changes of pyranine monitored at 450 nm of the wild-type RxR, and the E187Q, E197Q and E187Q/E197Q RxR mutants at 25 °C. The absorption changes of E187Q, E197Q and E187Q/E197Q were enlarged 4 times for comparison.

Back to article page