Figure 1 | Scientific Reports

Figure 1

From: Establishment of novel long-term cultures from EpCAM positive and negative circulating tumour cells from patients with metastatic gastroesophageal cancer

Figure 1

Characteristics of patient 20 tumour and UWG01CTC cell line. (A) Representative images of late passage (passage 40) UWG01CTC under hypoxic conditions in standard culture vessels with 10% FCS containing media (top image) or serum free media where they form loose spheroids (bottom image). Scale bar 50 µm. (B) UWG01CTC rapidly formed tumours in immunocompromised mice (n = 3), with all tumour endpoints reached within 3 weeks. (C) IHC analysis of UWG01CTC showing strong expression of neuroendocrine markers (CD56 and CGA), high Ki67 expression, but no expression of CSC markers (CD44, CD133, ALDH1). (D) IHC analyses of patient 20 tumour, patient derived cell line, mouse xenograft, and cell line derived from mouse xenograft (UWG01CTC-M) showing stable strong expression of the neuroendocrine marker synaptophysin, with consistent patchy cytokeratin positivity. Scale bar 100 µm.

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