Table 1 Expression, purification, and immobilizations of thermostable Tm-β-Glu from E. coli JM109 (DE3) harboring pET-28a-β-Glu.

From: Immobilization of β-Glucosidase from Thermatoga maritima on Chitin-functionalized Magnetic Nanoparticle via a Novel Thermostable Chitin-binding Domain

Recombinant proteins

Expressed activity (U mg−1)

Residual activity of the supernatant (%)

Yield (%)

purification (fold)

Free β-Glu

4.99a

100c

1.0a

Tm-β-Glu-Tt-ChBD-Ch-MNPs

58.06d

0.01a

99.9c

11.6d

Tm-β-Glu-SA-MNPs

42.86c

26.69c

73.31a

8.9c

Tm-β-Glu-Cs-MNPs

38.13b

21.27b

78.73b

7.6b

  1. *Activity was determined with 40 mM 4-Nitrophenyl-β-D-glucopyranoside (pNPG) as a substrate in 50 mM citrate buffer (pH 6.2) at 90 °C for 5 min.