Table 1 Expression, purification, and immobilizations of thermostable Tm-β-Glu from E. coli JM109 (DE3) harboring pET-28a-β-Glu.
Recombinant proteins | Expressed activity (U mg−1) | Residual activity of the supernatant (%) | Yield (%) | purification (fold) |
|---|---|---|---|---|
Free β-Glu | 4.99a | — | 100c | 1.0a |
Tm-β-Glu-Tt-ChBD-Ch-MNPs | 58.06d | 0.01a | 99.9c | 11.6d |
Tm-β-Glu-SA-MNPs | 42.86c | 26.69c | 73.31a | 8.9c |
Tm-β-Glu-Cs-MNPs | 38.13b | 21.27b | 78.73b | 7.6b |