Figure 2

Immunophenotyping bat BMDMs. Flow cytometry analysis of undifferentiated bat bone marrow cells (A–D, in green), M-CSF differentiated BMDMs (E–H, in purple) and GM-CSF differentiated BMDMs (I-L, in blue) demonstrating changes in FSC-A/SSC-A properties (A,E,I), CD11b expression (B,F,J), CD206 expression (C,G,K), CD206 and CD14 co-expression (D, H, L) during bat macrophage differentiation. Black contour plots indicate staining intensity in CD11b BV711 FMO stained controls. (M) Micrographs of Giemsa stained CD11b⁺ CD14⁺ CD206⁺ M-CSF BMDMs, and (N) CD11b⁺ CD14⁺ CD206⁺ GM-CSF BMDMs. (O) CD206 staining intensity in CD11b⁺ CD206⁺ CD14⁺ cells in bone marrow cells, and M-CSF and GM-CSF BMDMs. (P) Percentage of CD11b⁺ cells which stain positively for both CD14 and CD206 in bone marrow cells, M-CSF and GM-CSF BMDMs. Flow cytometry data and micrographs are representative of results from three individual bat derived tissues. Data in (O,P) is presented as mean ± SEM from three individual bat derived tissues. Scale bars represent 25 µm.