Figure 7
From: BAD sensitizes breast cancer cells to docetaxel with increased mitotic arrest and necroptosis
BAD requires ROS for docetaxel cell death. (a) MDA-MB-231 cells stably expressing vector or BAD were treated with 125 nM docetaxel or DMSO control for 48 hours prior to staining with CM-H2DCFDA to measure reactive oxygen species (ROS). Mean fluorescence intensity (MFI) was measured via flow cytometry. Fold increase of docetaxel/control is graphed. Student’s t-test; n = 3. (b) MDA-MB-231 cells stably expressing vector or BAD were treated with 125 nM docetaxel or 10 mM N-acetyl cysteine (NAC) for 5 days. Annexin V+/PI+ staining flow cytometry analysis of cells is graphed. Two-way ANOVA with Tukey’s post-hoc test; n = 3. (c) MDA-MB-231 cells expressing BAD were treated with 125 nM docetaxel or 50 nM rotenone for 5 days. Annexin V+/PI+ staining flow cytometry analysis of cells is graphed. Student’s t-test; n = 3. (d) Schematic representation of docetaxel-mediated cell death. BAD increases length in mitotic arrest by inhibiting cyclin B1 degradation, leading to a ROS-dependent necroptotic cell death.
