Figure 3
DSC measurements. DSC curves of a-miR-23 (A) and a-miR-141 (B) of unfolding dependence on strand concentration (0.18, 0.24 and 0.40 mg/mL) in 10 mM PBS, 100 mM NaCl at pH 6.8. DSC curves (black open circles) are deconvoluted in two components: the red and green curves correspond to the 1st and 2nd transition of a biphasic melting curve. The sum of the two components is reported as a blue line. (C) Cartoon representations of possible structures (self-dimers and hairpins) of a-miR-23 and a-miR-141, calculated by “IDT® oligoanalyzer tool”.
