Figure 5
PAGE experiments. PAGE of a-miR-23 and a-miR-141 performed 72 h after incubation at 37 °C with DMEM buffer (lanes 4 and 6, respectively) or DMEM + 10% FBS (lanes 5 and 7, respectively). The PAGE behaviour of the control T24 RNA sequence in the absence or presence of FBS nucleases is shown in lanes 2 and 3, respectively. All samples were loaded at 175 µM concentration in the same run. The running marker bromophenol blue was loaded in lane 1.
