Figure 2

HDAC10 deletion does not impair conventional T cell function. (A–C) WT and HDAC10^−/− conventional T cells were co-stimulated and cultured under polarizing conditions to form Th1 (A), Th17 (B), and induced Treg (C,D). HDAC10^−/− Tconv showed a trend to form less Foxp3⁺ induced Treg, but significance was missed (Wilcoxon matched-pairs signed rank test). Data representative of two (A,B) and five (C,D) independent experiments. (E–H) Parent-to-F1 assay. (E) schematic: 4 × 10⁷ C57BL/6 (H-2^b) WT or HDAC10^−/− splenocytes were CFSE-labeled, and adoptively transferred (i.v.) into C57BL/6-DBA/2 (H-2^bd) recipients. After three days, the adoptively transferred cells were identified by their absence of H-2^d MHC. CD8⁺ and CD4⁺ T cells lacking HDAC10 proliferated equally well compared to WT in vivo. (F) Gating strategy. (G,H) Pooled data of CD4⁺ and CD8⁺ T cell proliferation (G) and cytokine production after PMA/ionomycin activation (H). Data pooled from three independent experiments (Mann-Whitney test). Abbreviations: n.s., not significant. Data shown as median ± IQR.