Figure 3

HDAC10^−/− Tregs have increased suppressive function. CD4+ CD25⁻ T cells were CFSE-labeled and co-stimulated with anti-CD3 mAb and WT irradiated CD90.2⁻ antigen presenting cells. Regulatory T cells (TR) were added to the stimulated T-effector (TE) cells at the indicated TR:TE ratio. After three days, proliferation of the co-stimulated T-effector cells was assessed via flow cytometry by measuring CFSE dye dilution. We combined TE, CD90.2⁻ and TR from WT or HDAC10^−/− mice. (A) Representative comparison of WT versus HDAC10^−/− TR suppressive fuction. (B) Quantitative TR suppressive function data pooled from 12 WT versus HDAC10^−/− TR pairings from 8 independent experiments experiments (paired Student t-test, * indicates p < 0.05). (C) Representative and (B) quantitative comparison of six WT versus HDAC10^−/− CD90.2⁻ pairings from two independent experiments (Wilcoxon matched-pairs signed rank test). (B) Data shown as mean ± SEM. (D) Data shown as median ± IQR.