Figure 2
Global proteome analysis of hiPSC differentiating either on Matrigel or encapsulated in alginate capsules. (a) Scheme illustrating the cell populations and differentiation stages considered for global proteomics. (b) Experimental design of the conditions compared in TMT 11-plex proteomics. (c) Hierarchical clustering of normalized TMT-ratios (n = 2, 2, 2, 2, 3). (d) Analysis workflow depicting the comparisons employed and the assessed corresponding effect. (e) The number of proteins showing a dynamic of regulations compatible with an islet-promoting pattern in response to each of the four effects considered. Arrows depict the generic prerequisite direction of regulation for group inclusion. (f) Pies charts depicting the proportion of proteins following islet-promoting and islet-antagonizing regulation patterns in each of the four effects considered. The graph bars represent the proportion of proteins reaching abundance levels indistinguishable from those detected in native human islets.
