Figure 2
From: Statin use, HMGCR expression, and breast cancer survival – The Malmö Diet and Cancer Study
Validation of anti-HMGCR antibodies. (A–D) Western blots showing the expression of HMGCR after respective treatments tracked by different antibodies. All western images are full-length blots. (A) HMGCR AMAb90619, (B) HMGCR AMAb90618, (C) HMGCR A-9 and (D) HMGCR ab174830. Human breast cancer MCF-7 cell line was the main test cell line, while the HEPG2 liver cancer cell line and the Chinese hamster ovary cell lines CHO-K1 and UT-1 (derived from CHO-K1 following prolonged exposure to mevastatin) served as additional controls. (E) RT-qPCR was performed to evaluate the efficiency of downregulating HMGCR with siRNA or upregulating HMGCR with statin treatment. (F) Correlation of gene- and protein expression measured by antibody AMAb90619 in tumors. Figure re-used from thesis of Olöf Bjarnadottir20.
