Figure 1

Expression of ChR2(H134R) at the hypoglossal motoneuron pool. Fluorescent microscopy images illustrating the expression of ChR2(H134R)-EYFP and ChAT in coronal brain sections. Shown are low-magnification (left column) and high-magnification (right column) representative images taken from a ChAT-ChR2(H134R)-EYFP mouse at 7.4 mm posterior to bregma stained for DAPI, EYFP and ChAT. The colocalization of EYFP and ChAT at the hypoglossal motoneuron pool is also illustrated (‘merge’).