Figure 1

HMV induces lung injury in mice and reduces FAK expression in AECs. Treatment group mice underwent 4 h HMV (VT 30 ml/kg, respiratory rate of 75 breaths/min, positive end-expiratory pressure of 0 cm H2O), while control mice were maintained with spontaneous breathing. Then, lung permeability was assessed as representative images (A) of lung tissue (H&E, 100×) shown. The quantification of lung injury (B), the lung wet/dry ratio (C), BALF protein level (D) and BALF cell counts (E) in the harvested lung tissues. (F) HMV exposure reduced FAK expression in the mouse lung (IHC, 400×). (G) HMV exposure reduced phospho-Akt expression in the mouse lung (IHC, 400×). The data are presented as the mean ± SEM (*p < 0.05; **p < 0.01 by two-tailed t test). control, without mechanical ventilation; HMV, high-volume mechanical ventilation.