Figure 3

Impact of high glucose conditions on Pdgfb expression in macrophages. (a) Pdgfb mRNA expression levels in peritoneal macrophages treated with 100 ng/mL LPS or 10 ng/mL IL-4 under 11 mM glucose for 24 hours. n = 4 (b) Representative scatter plot of living CD45⁺F4/80⁺ peritoneal macrophages and a histogram of 2-NBDG in these cells by flow cytometry. (c) Relative changes in the mean fluorescent intensity (MFI) of 2-NBDG in living CD45⁺F4/80⁺ peritoneal macrophages treated with 100 ng/mL LPS for 24 hours. n = 3. (d–f) Relative expression levels of Itgax, Mrc1, and Pdgfb mRNA in RAW264.7 cells treated with several glucose concentrations for 24 hours. Mean of data among six independent experiments. n = 14–15. (g) Relative expression levels of Pdgfb mRNA in RAW264.7 cells treated with 100 ng/mL LPS for 3 hours under several glucose concentrations. Mean of data among three independent experiments. n = 6. (h) Relative lactic acid contents in the cultured medium of RAW264.7 cells treated with 100 ng/mL LPS for 3 hours under several glucose concentrations. Mean of data among three independent experiments. n = 4. Data are shown as means ± S.E. *p < 0.05 and **p < 0.01, among two groups, as indicated. A.U., arbitrary unit.