Figure 5
From: Differential interaction of Or proteins with the PSY enzymes in saffron
CsOr-a interacts with CsPSY enzymes. Yeast two-hybrid assays for CsOr-a and CsOr-b interaction with CsPSYs and CsCCD2. CsOr-a or CsOr-b was fused to the activating domain (AD), and CsPSY1a, CsPSY1b CsPSY2, CsPSY3 or CsCCD2 was fused to the binding domain (BD). (a) Yeast cells transformed with different combinations of constructs were grown on a chromogenic selective medium (SD/-Trp/-Leu/X-α-Gal/AbA). (b) Yeast cells transformed with different combinations of constructs of Or and PSY proteins were spotted on selective medium (SD/-Ade/-His/-Leu/-Trp), supplemented with Aureobasidin A (AbA). (c) β-galactosidase assays of in vivo interactions between CsOr-a and CsPSY and CsCCD2 proteins. Means and se from three biological replicates are shown.
