Figure 2
From: Novel invasion indices quantify the feed-forward facilitation of tumor invasion by macrophages
HT1080 spheroid growth and characterization. (A) A series of micrographs showing a spheroid growth in a hanging drop for days 1–3 and the low binding plate for days 4–6. (B) Image processing flow to calculate spheroid area and volume. The plot profile was obtained from the rectangle (yellow line) across the center of the spheroid and the threshold value (red dotted line) was set smaller than the background signal. After converting the image to binary, the area of the spheroid was obtained. (C) The cell numbers estimated by DNA content and the volume calculated by using (B) display a good correlation. The HT1080 spheroid grows rapidly for the first three days, then it continues growing with a reduced growth rate. Scale bar = 250 µm.
