Figure 5 | Scientific Reports

Figure 5

From: Novel invasion indices quantify the feed-forward facilitation of tumor invasion by macrophages

Figure 5

LLC1 spheroid growth and characterization. (A) Daily micrographs showing the spheroid growth in a hanging drop (for the first three days) and low binding plate (latter two days). Scale bar = 250 µm. (B) Daily plots for volume and roundness of spheroid. (C) Daily histograms of spheroid diameter (initial cell number = 1,000) shows that the size of spheroids remains uniform throughout the culture. (D) The plot between cell number and the calculated volume.

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