Figure 1 | Scientific Reports

Figure 1

From: Kidney transplant monitoring by urinary flow cytometry: Biomarker combination of T cells, renal tubular epithelial cells, and podocalyxin-positive cells detects rejection

Figure 1

Establishment of a staining assay using human kidney tissue to analyze tubular epithelial cells and podocalyxin-positive cells by flow cytometry. (A) Kidney tissue staining. Human kidney tissue from deceased patients was used to establish an applicable antibody panel. TEC biomarker Cytokeratin (intracellular) (grey: unstained, blue: Cytokeratin). Cytokeratin+ cells were used to differentiate between proximal (CD10+, blue) and distal (EPCAM+, blue) TEC; Isotype controls (grey). Podocytes stained with PDX and PDX isotype. (B) Urinary isotype controls for TEC and podocytes. Cytokeratin+ (intracellular) TEC stained with CD10 and EPCAM; isotype controls for cytokeratin, CD10 and EPCAM. Podocytes stained with PDX and PDX-Isotype. TEC, tubular epithelial cells; PDX, podocalyxin; EPCAM, epithelial cell adhesion molecule.

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