Table 1 Composition of the experimental diets.

From: Nicotianamine-chelated iron positively affects iron status, intestinal morphology and microbial populations in vivo (Gallus gallus)

Diet Ingredient

Control

Biofortified

g/Kg (by formulation)

Control white wheat flour

800

Biofortified white wheat flour

800

skim milk, dry

99.75

99.75

DL-methionine

2.5

2.5

corn oil

27

27

choline chloride

0.75

0.75

vitamin/mineral premix (no Fe/Zn)

70

70

Selected Components

Control

Biofortified

Dietary Fe (µg/g)

25.9 ± 0.12

28.9 ± 0.13***

Dietary Zn (µg/g)

16.6 ± 0.06

19.2 ± 0.03***

Dietary NA (µmol/g)

18.1 ± 0.32

33.0 ± 0.21***

Dietary DMA (µmol/g)

19.5 ± 0.16

34.1 ± 0.74***

Dietary Phytate (mg/g)

0.5 ± 0.09

0.5 ± 0.08

Total Fiber (µg/g)

19.9 ± 0.18

23.8 ± 1.12

Total Protein (%)

13.47 ± 0.08

13.67 ± 0.08

Total Carbon (%)

41.90 ± 0.13

41.30 ± 0.13

Phytate: Fe molar ratio

1.63

1.46

  1. Component values represent mean ± SEM of at least four technical replicates. Asterisks denote significant differences for p ≤ 0.001 (***) as determined by Student’s t-test.