Table 2 Primers and related information of the candidate reference genes and the target gene.
Gene | Primer pairs (5′-3′) | Efficiency (%) | Coefficient of determination (r2) | product size (bp) |
|---|---|---|---|---|
GAPDH | F: GTATTGGCCGTCTGGTCCT R: GGGAGACCTCACCGTTGTAA | 98.0 | 0.997 | 144 |
18S | F: GTGGAGCGATTTGTCTGGTT R: CTCAATCTCGTGTGGCTGAA | 96.5 | 1.000 | 130 |
ACTB | F: CATGTACGTTGCCATCCAAG R: ACCAGAGGCATACAGGGACA | 104.9 | 0.996 | 138 |
EF1A | F: CGGCCACCTGATCTACAAGT R: GCCTTCAGTTTGTCCAGCA | 90.9 | 0.997 | 123 |
CTSD | F: GAAGAAGGTGGAGCAGAACG R: TGCGGGTGATGTTGATGTAG | 96.8 | 0.999 | 137 |
B2M | F: GGCAGTTCCATCTGACCAAG R: ATGTTTGACTCCCAGGCGTA | 92.8 | 0.998 | 112 |
ERα | F: GCCACCACTATCTGGAAACC R: CCTGACTCCCCCAAACTGTA | 90.9 | 0.996 | 115 |
