Table 1 Overview of used antibodies and markers.
Name | Abbreviation | Host species | Dilution | Used to label | Vendor/Donor (catalogue #, batch #, RRID/CAS #) | References/Specificity |
|---|---|---|---|---|---|---|
4′,6-diamidin-2-phenylindol | DAPI | 1:20,000 | Cell nuclei (DNA) | Sigma Aldrich, Steinheim, Germany (D9542, n/a, 28718-90-3) | ||
5-ethynyl-2′-desoxyuridine | EdU | 100 µM | newly born cells (newly synthesized DNA) | Thermo Fischer Scientific, Rockford, IL, USA (A10044; 1259424; 61135-33-9) | ||
Alexa Flour 488 coupled phalloidine | Phalloidin | 1:200 | Ubiquitous neuroanatomy (F-Actin) | Thermo Fischer Scientific, Rockford, IL, USA (A12379; n/a; n/a) | ||
Alexa Flour 488 Azide | 488-azide | 1 µM | 5-ethynyl-2′-desoxuridine | Thermo Fischer Scientific, Rockford, IL, USA (A10260; 1320994; n/a) | ||
Anti-Drosophila melanogaster reversed polarity (4α3) | anti-repo | Rabbit | 1:5,000 | Glia cells | B. Altenhain, University of Mainz, Germany (n/a; n/a; n/a) | |
Anti-Red fluorescent protein | RFP | Chicken | 1:3,000 | DsRed | Rockland Immunochemicals INC, Limerick, PA, USA (600-901-379, 26274, AB_10704808) | |
Cy3 coupled goat anti-chicken | GACh-Cy3 | Goat | 1:300 | Primary antibodies raised in chicken | Jackson ImmunoResearch; Westgrove, PA, USA (103-165-155, 93117, AB_2337386) | |
Cy3 coupled goat anti-rabbit | GAR-Cy3 | Goat | 1:300 | Primary antibodies raised in rabbit | Jackson ImmunoResearch; Westgrove, PA, USA (111-165-144, n/a, AB_2338006) | |
Cy5 Azide | Cy5-azide | 1 µM | 5-ethynyl-2′-desoxuridine | Jena Bioscience, Jena, Germany (CLK-CCA-9295-1; Kli008-078; n/a) | ||
Cy5-Sulfo Azide | 1 µM | 5-ethynyl-2′-desoxuridine | Jena Bioscience, Jena, Germany (CLK-AZ118-1; Kli009-030; n/a) | |||
Moth-R2, Orco antiserum | Moth-R2 | Rabbit | 1:5,000 | Olfactory sensory neurons | J. Krieger, University Halle-Wittenberg, Germany (n/a; n/a; n/a) |
