Figure 2
From: Interactions of Cisplatin and Daunorubicin at the Chromatin Level
Effect of Cis-Pt on Dauno uptake. Total cellular (a) and nuclear (b) fluorescence intensity distributions of Dauno, and of Hoechst 33342 (c), measured by flow-cytometry. Fluorescence intensity distributions of 20,000 events were recorded in a representative experiment. (a,b) Distribution curves of Dauno fluorescence of HeLa cells (a), or nuclei (b), after treatment of live cells with 1.5 μM Dauno only (red line), with 1.5 μM Dauno in combination with 40 μM Cis-Pt (green), or left untreated (black). (c) Hoechst 33342 fluoresence intensity distributions of live HeLa cells. The Hoechst dye (H) was added to the cells at 35 μM concentration following incubation with Dauno and/or Cis-Pt at different combinations: Dauno and Hoechst (red), Dauno, Cis-Pt and Hoechst (green), Hoechst 33342 only (blue), Hoechst 33342 and Cis-Pt (turquoise). The table inset shows the decrease of the mean Hoechst fluorescence in the samples co-treated with Dauno (D + H), Dauno + Cis-Pt (D + C + H) and Cis-Pt only (C + H), in % of the control (blue).
