Figure 3
From: A high sensitivity ZENK monoclonal antibody to map neuronal activity in Aves
The 7B7-A3 antibody is a neuronal activity marker. (a) Set-up for auditory activation experiment. Pigeons were immobilized in a body harness and exposed to high frequency sounds delivered through two speakers at 1.5 m distance to the animals (n = 6) or to no sound (n = 6, Control). (b) Sound stimulation paradigm: T1-t50: double-pulsed pure tones and sweeps (each 50 ms, 2–10 kHz, 70 dB SPL), delivered in a pseudorandomized order; t51-t60: silence; t61-t90: white noise (2–10 kHz, 70 dB SPL); t91-t120: silence. The sound file was looped 30 times to achieve a 60 min stimulation. (c–e) Quantification of ZENK+ cells per mm2 in the nucleus angularis, (f–h) nucleus magnocellularis, (i–k) nucleus ventralis lemnisci lateralis (VLV), and (l–n) spinal trigeminal nucleus (SpV). Right panels show representative coronal sections from control and stimulated animals stained with the 7B7-A3 antibody as well as the segmented regions for the nucleus angularis (d,e), nucleus magnocellularis (g,h), VLV (j,k), and SpV (m,n). On the left of (d), (g), (j), and (m), anatomical drawings of coronal hemisections with the segmented nuclei are shown in orange and stereotaxic coordinates according to Hodos and Karten50 are indicated in the grey boxes. Data are presented as mean +/− SD. *P < 0.05; ***P < 0.0001, one tailed t-test after Bonferroni-correction in auditory areas. n.s.: not significant. Scalebars represent  2 mm in the drawings and 200 μm in stained sections.
