Figure 5
Validation in Erlenmeyer flasks of active compounds highlighted in the pilot screening. Treatment with 1 µM TSA was re-tested in standard 250-ml Erlenmeyer flasks with an exposure time varying between 1, 7 and 14 days. (a) Embryo yield (embryos. g−1 callus) obtained after 9 weeks. (b) Biomass (g) obtained after 9 weeks. (c) Embryo length. Bars show the mean of triplicate samples (n = 6 flasks/exposure time/replicate for (a,b) and n = 100 embryos/exposure time/replicate for (c)) and error bars represent SD. The “CTRL” group was untreated (control). Differences between treated clusters and the control were analysed with a one-way ANOVA test for (a,b), while a Kruskal-Wallis test was used for (c) followed by a Dunn test as a post hoc. Means with different letters are significantly different (P < 0.001).
