Figure 3 | Scientific Reports

Figure 3

From: Human liver stem cells express UGT1A1 and improve phenotype of immunocompromised Crigler Najjar syndrome type I mice

Figure 3

HLSC injection in NSG wt mice. (A) Immunofluorescence analysis of DiI-labelled non-injected control liver is shown in i; DiI-labelled HLSC in a 2 month old wt mouse liver after partial hepatectomy (ii, iii). (B) Flow cytometry analysis of 21 days old NSG mouse livers injected with (i) PBS and (ii) HLSC at Day 5. Each panel is representative of three independent experiments (n = 3). (iii) The graph shows percentage of HLSC engraftment (n = 3). (C) Liver of 21 days old pups injected at Day 5 either with PBS or with DiI-labelled (red) HLSC and stained for UGT1A1 (Alexa488, green) and DAPI (blue) (for higher magnification see Fig. S3) (upper panel). Quantification of the percentage of UGT1A1 expressing cells in the DiI positive population (lower panel); HLSC injected liver not stained for UGT1A1 was used as control. (n = 3).

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