Figure 2 | Scientific Reports

Figure 2

From: Shotgun proteomics, in-silico evaluation and immunoblotting assays for allergenicity assessment of lesser mealworm, black soldier fly and their protein hydrolysates

Figure 2

(A) SDS-Page of: black soldier fly (BSF) and lesser mealworm (LM) larvae; (B) IgG-immunoblotting of the samples separated by SDS-Page, followed by incubation with anti-tropomyosin IgG; (C) IgE-immunoblotting results after incubation with sera from non-allergic patient and person allergic to crustacean tropomyosin. Response from IgG binding can be visible in a and b, while from IgE binding in c–g. M: marker. Full-length blots/gels are presented in Supplementary Figs. S1–S3.

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