Figure 3 | Scientific Reports

Figure 3

From: Shotgun proteomics, in-silico evaluation and immunoblotting assays for allergenicity assessment of lesser mealworm, black soldier fly and their protein hydrolysates

Figure 3

(A) SDS-Page of: protein hydrolysates obtained from proteolysis activity of the protease from Bacillus licheniformis on black soldier fly (BSF-h) and lesser mealworm (LM-h) protein hydrolysates; (B) IgG-immunoblotting of the samples separated by SDS-Page, followed by incubation with anti-tropomyosin I IgG; (C) IgE-immunoblotting results after incubation with sera from non-allergic patient and person allergic to crustacean tropomyosin. Response from IgE binding in a and b. M: marker. Full-length blots/gels are presented in Supplementary Figs. S4–S8.

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