Figure 4 | Scientific Reports

Figure 4

From: Thermostabilization of VPR, a kinetically stable cold adapted subtilase, via multiple proline substitutions into surface loops

Figure 4

Normalized melting curves of proline variants in glycine buffer containing 15 mM CaCl2 and 100 mM NaCl. VPRΔC (black dotted line). (A) Melting of VPRΔC_N3P (Gold) and VPRΔC_I5P (purple). (B) Melting of VPRΔC_N238P (light blue) and VPRΔC_T265P (orange). (C) Melting of VPRΔC_N3P/I5P/N238P (blue) and VPRΔC_N3P/I5P/T265P (green). (D) Melting of VPRΔC_N3P/I5P (red) and VPRΔC_N3P/I5P/N238P/T265P (black).

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