Figure 5 | Scientific Reports

Figure 5

From: Thermostabilization of VPR, a kinetically stable cold adapted subtilase, via multiple proline substitutions into surface loops

Figure 5

Arrhenius plots calculated form the thermal inactivation of proline variants in Tris buffer containing 15 mM CaCl2. VPRΔC (black boxes with a dotted black line). (A) Thermal inactivation of VPRΔC_N3P (Gold) and VPRΔC_I5P (purple). (B) Thermal inactivation of VPRΔC_N238P (light blue) and VPRΔC_T265P (orange). (C) Thermal inactivation of VPRΔC_N3P/I5P/N238P (blue) and VPRΔC_N3P/I5P/T265P (green). (D) Thermal inactivation of VPRΔC_N3P/I5P (red) and VPRΔC_N3P/I5P/N238P/T265P (black).

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