Table 1 Kinetic parameters of VPRΔC and proline variants. Values are expressed as the averages and the standard deviations of the mean.

From: Thermostabilization of VPR, a kinetically stable cold adapted subtilase, via multiple proline substitutions into surface loops

Variant

kcat

(s−1)

Km

(mM)

kcat/KM

(s−1mM−1)

VPR∆C

225.7 ± 12.0

0.177 ± 0.016

1238 ± 149

VPR∆C/N3P

235.4 ± 21.8

0.173 ± 0.013

1364 ± 60

VPR∆C/I5P

201.6 ± 8.2

0.187 ± 0.010

1077 ± 37

VPR∆C/N238P

224.6 ± 16.6

0.189 ± 0.026

1196 ± 84

VPR∆C/T265P

166.5 ± 11.6

0.152 ± 0.019

1101 ± 104

VPR∆C/N3P/I5P

231.8 ± 10.5

0.187 ± 0.009

1243 ± 77

VPR∆C/N3P/I5P/N238P

229.5 ± 18.3

0.199 ± 0.024

1158 ± 72

VPR∆C/N3P/I5P/T265P

221.5 ± 7.8

0.219 ± 0.014

1017 ± 74

VPR∆C/N3P/I5P/N238P/T265P

259.3 ± 27.4

0.212 ± 0.014

1222 ± 95

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