Table 2 Relative intensity of fluorescence calculated as area under the curve (AUC), the maxima of curves (λmax) and the Stern-Volmer constant at 25 °C and pH 8.0 of VPRΔC and the proline variants.

From: Thermostabilization of VPR, a kinetically stable cold adapted subtilase, via multiple proline substitutions into surface loops

Variant

Stern-Volmer

(M-1)

λmax

(nm)

Relative intensity

VPR∆C

2.24 ± 0.12

335 ± 1

1.00 ± 0.03

VPR∆C/N3P

2.32 ± 0.18

337 ± 2

1.37 ± 0.02

VPR∆C/I5P

2.05 ± 0.13

337 ± 2

1.16 ± 0.05

VPR∆C/N238P

2.63 ± 0.25

339 ± 2

1.16 ± 0.07

VPR∆C/T265P

2.41 ± 0.14

338 ± 1

1.15 ± 0.08

VPR∆C/N3P/I5P

1.64 ± 0.07

334 ± 1

1.00 ± 0.03

VPR∆C/N3P/I5P/N238P

2.18 ± 0.05

339 ± 1

1.18 ± 0.08

VPR∆C/N3P/I5P/T265P

2.17 ± 0.05

338 ± 1

1.24 ± 0.03

VPR∆C/N3P/I5P/N238P/T265P

2.12 ± 0.19

336 ± 2

1.19 ± 0.06

  1. Values are expressed as the averages and the standard deviations of the mean.
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