Table 5 Sequences of mutagenic primers used.
Primer | Sequence |
|---|---|
N3P fw. | CGTTCAAAGCCCGGCGATTTGGG |
N3P rv. | GCTTCATTTGAAACAACAG |
I5P fw. | GTTCAAAGCAACGCGCCGTGGGGGCTAGACCG |
I5P rv. | CGGTCTAGCCCCCACGGCGCGTTGCTTTGAAC |
N3P/I5P fw. | GCCGTGGGGGCTAGACCGAATA |
N3P/I5P rv. | GCCGGGCTTTGAACGGCTTCATTTG |
N238P fw. | ACAAGAAAACCCGGGCTTAACTCCGCTTC |
N238P rv. | AAGTACAAGGCTGCAACG |
T265P fw. | AAGAGGCACCCCGAATAAACTGC |
T265P rv. | GTGTCAGAAACCTTATTCTC |
