Figure 5 | Scientific Reports

Figure 5

From: Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences

Figure 5

Improvement in optical resolution. (a) Widefield (WF) and (b) reconstructed images of orange fluorescent beads, 100 nm in diameter. (d) WF and (e) SR-SIM images from gene-edited U2OS cells, labelled with EGFP. The intensity of the outlined regions in (a,b) were subjected to a non-linear surface fitting with a Gaussian 2D function, allowing to compare the PSFs in (c). Normalized intensity profiles from the arbitrary cross-sections in (d,e) were subsequently fit with a 1D Gaussian function to allow direct correlation of the FWHM for each sample in (f). Scalebars in (a,b) and (d,e) correspond to 1 μm and 2 μm respectively.

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