Figure 2
From: Capsid-specific removal of circulating antibodies to adeno-associated virus vectors
AAV-specific column for the removal of anti-capsid IgG from plasma. (a) Diagram of the column. AAV capsid particles are grafted to the column matrix and allow for binding and removal of IgG specific to the capsid without affecting total antibody levels. (b) Anti-AAV8 IgG retention rate of IVIg and plasma at various concentrations and volumes. A flow rate of 0.1 ml/minute was used in all experiments. For the IVIg retention experiment, n = 3 runs. For 1 ml and 5 ml plasma at 1/7290 dilution, n = 5 runs; shown is average, error bars represent the standard error of the mean. (c) Effect of AAV capsid loading on anti-AAV8 IgG retention. Various amounts of AAV8 capsid were used to bind to the NHS-Sepharose matrix and retention of AAV8 IgG measured after IVIg or seropositive plasma loading. (d) Effect of flow rate on anti-AAV8 IgG retention. AAV8 IgG retention tested with 3 columns containing various amounts of AAV8 capsid at 3 different flow rates.
