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Figure 1

From: Zika virus infection leads to mitochondrial failure, oxidative stress and DNA damage in human iPSC-derived astrocytes

Figure 1

ZIKV infection rate of human brain cells derived from iPSC. (A–C). Cells were infected at a multiplicity of infection (MOI) = 1 and analyzed 72 hours post infection (hpi). (A). Immunofluorescence images of NSCs stained for ZIKV NS1 protein (green) and Nestin (red). (B) Astrocytes stained for ZIKV NS1 protein (green) and S100b (red). (C) Neurons stained for ZIKV NS1 protein (green) and beta tubulin III (red). Blue channel is DAPI staining for all cell types. Scale bar: 50 µm. (D). Quantification of the rate of infection according to cell type (Data is shown as average ± SEM. For NSCs, n = 3, derived from iPS lines GM23279A, CF1 and CF2, *p = 0.026; for astrocytes, n = 4, derived from iPS lines GM23279A, CF1, CF2 and C15, **p = 0.003; for neurons, n = 3, derived from iPS lines GM23279A, CF1 and CF2, non-significant). (E) Cell viability assay (MTT) for MOCK and ZIKV-infected NSCs, non-significant, n = 3. (F) MTT for MOCK and ZIKV-infected astrocytes, n = 3, ***p < 0.001. Data is shown as average ± SEM.

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