Figure 5
From: Tannic acid inhibits lipid metabolism and induce ROS in prostate cancer cells
Tannic acid interferes with Lipid biogenesis and metabolism in prostate cancer cells. (A) FTIR spectral analysis of lipid content as extracted from C4-2 and PC-3 cells after TA treatment by Bligh and Dyer protocol. (B) The percent lipid ratios (ester/methyl and ester/-C-H) of cellular lipid extracts in C4-2 and PC-3 cells. Values obtained from the FTIR measurements (n = 6). (C) Western blot analysis showing dose dependent TA treatments affects the gene expression related to lipogenic signaling pathway as demonstrated by lipogenic proteins such as Lipin 1, Fatty acid synthase (FASN), Acetyl-CoA carboxylase (ACC), Cytoplasmic acetyl-CoA synthetase (ACeCS1) and Mammalian long-chain acyl-CoA synthetase (ACSL1). The protein ladder used for these bolts’ ranges from 10 kDa to 250 kDa (Bio-rad cat#1610374, Hercules, CA). All full-length blots are presented in Supplementary Information. (D) Heat map analysis of lipid metabolism related genes during TA treatments on C4-2 and PC-3 cells. Data presented as mean ± standard error of the mean (n = 3). * and *** Represent level of significance with P (<0.05 and 0.001) with respect to control.
